CXCL8 and Sepsis: We therefore cultivated primary human neutrophils (viability 89.6%) from healthy volunteers (n = 8) and incubated the cells for 8 h with either 10% serum from healthy controls, IL-8 (100 ng/ml) or macrophage supernatant (to activate neutrophils) as well as with 10% serum from patients with sepsis (Fig. 4a).