Notably, while any of the five previously validated lysate markers of DEN-induced cancer were not successful in discrimination of HCC from control tissue, two markers from mitochondria-enriched data (i.e. Ndufb9 and Ndufs4) and two based on mtFE score values (i.e. Lonp1 and Ogdh) confirmed their differential expression and high prediction power to cancer samples (e.g. AUCs Lonp1 and Ogdh mtFE scores > 0.8, Fig. 7b,c). This evidence concerns the gene NDUFS4 and hepatocellular carcinoma.