In the present study, based on the significant correlation between high miR-130b expression and poor overall survival in patients with NSCLC, we demonstrated that miR-130b increased invasion activity by directly targeting TIMP-2 not only in adenocarcinoma cells but also in squamous cell carcinoma cells using loss-of function and gain-of function experiments. This evidence concerns the gene TIMP2 and non-small cell lung carcinoma.