Moreover, prompted by the evidence that SGI-1027 induced a selective downregulation of DNMT1 with minimal or no effects on DNMT3A in different cancer cell lines [19], and that the DNMTi MC3343 decreased the expression of all DNMTs in primary osteosarcoma cells [22], we tested MC3353 and the reference drug SGI-1027, in HCT116 and PC-3 cells to evaluate the putative MC3353 ability to provide a similar effect. Here, DNMT3A is linked to osteosarcoma.