As shown in Figure 3, we observed that mRNA expressions of CDK4 (see Figure 3B), TWIST1 (see Figure 3C), and SNAI1 (Snail) (see Figure 3D) were significantly upregulated in metastatic prostate cancer tissues compared to the primary tumor group, whereas mRNA expressions of the CCND1 (Cyclin D1) (see Figure 3A), and CDH1 (E-cadherin) (see Figure 3F), show no statistically significant difference between primary prostate cancer tissues and metastatic prostate cancer tissues (p = 0.277 for CCND1; p = 0.440 for CDH1). This evidence concerns the gene TWIST1 and prostate cancer.