EGF and breast cancer: To evaluate the applicability of the chips to explore challenging cell behaviors that unfold within a few minutes, SKBR3 breast cancer cells, which overexpress HER2 receptors, were loaded on a transversal stimulation chip (Figs. 7a, 8a, b), exposed to 3 min stimulation with EGF (10 nM) at 1 μL/min (Fig. 7b), flushed out quickly with hypotonic buffer (<1 min), and subjected to off-chip sonic cell lysis.