However, as reported in the previous studies, S473 phosphorylation on AKT automatically increased in NICD activated cells at the 72th h after transfection (Zhang et al., 2012; Wang et al., 2014), and suppression of Notch1 downregulated AKT phosphorylation/activation in breast cancer cells at 48th h after transfection (Li et al., 2016). The gene discussed is NOTCH1; the disease is breast cancer.