The results of flow cytometry using CD56+CD45+CD3- revealed that the ratio of NK-92MI to tumor cells in the tumor was 1.69 ± 0.32%, which was similar to two other studies the NK cell to brain tumor cell ratio in the blood-brain barrier (BBB) opening with UTMD is 1:100 (Alkins et al., 2013a) whereas the NK cell to human colorectal adenocarcinoma cell ratio in blood tumor barriers opening with UTMD is 1.21 ± 0.32% (Sta et al., 2015). This evidence concerns the gene NCAM1 and brain neoplasm.