Furthermore, despite combination with cisplatin and miR-124 induced significant apoptosis of CD133+ HepG2 and Huh7 cells, we showed that either SIRT1 plasmid or NAC can protect these HCC cells from the cytotoxicity of cisplatin and miR-124 co-treatment (Figure 5C). This evidence concerns the gene SIRT1 and hepatocellular carcinoma.