In our study, we analyzed the common clinicopathological features including age, BMI, location of tumor, T-stage, local infiltration, N-stage, histology type, ER expression and PR expression in HER2-positive and -negative cohort, and found that ER negative, PR negative, T1 stage and N2/N3 stage were independent risk factors for HER2 positive breast cancer. The gene discussed is ESR1; the disease is breast cancer.