This adaptive immune readout involved pulsing autologous monocytes (capable of antigen processing and presentation) with 15-mer overlapping peptides of TAA (melanocyte protein PMEL [PMEL], tyrosinase [TYR], and melanoma antigen recognized by T cells 1 [MART-1/MELAN-A]) and co-culturing these with CTLs. This evidence concerns the gene PMEL and melanoma.