Notably, it was reported that the overexpression of secreted Sema3A and Sema3F in human GBM cells U87MG dramatically reduced their proliferation, as well as colony formation in soft agar, while no significant variations were observed in culture upon expression of the homologous family members Sema3B, Sema3D, and Sema3G [26]. This evidence concerns the gene SEMA3D and glioblastoma.