DIRAS3 and ovarian carcinoma: When CEBPα was knocked down with siRNA, we observed that nutrient deprivation was no longer able to upregulate DIRAS3 mRNA expression across several ovarian cancer cell lines (Figure 7A–C) and this correlated with a decrease in autophagy induction as determined by immunofluorescence staining of LC3 puncta (Figure 7A–C).