SQSTM1 and ovarian carcinoma: When A2780 ovarian cancer cells were grown in media lacking amino acids (amino acid starvation) or in serum-free Hanks buffered salt solution (HBSS) supplemented with 3% glucose for 0–24 h (serum starvation), western blot analysis demonstrated a decrease in p62 (SQSTRM) and conversion of LC3-I to LC3-II, two established markers of autophagy [16] (Figure 1A,B).