Downregulation of E2F1/4 correlated with the induction of autophagy and upregulation of DIRAS3 protein determined by immunofluorescence staining of LC3 puncta and DIRAS3 protein in A2780, SKOv3, and ES2 ovarian cancer cells (Figure 4E–G). The gene discussed is DIRAS3; the disease is ovarian carcinoma.