Western blotting of whole-cell lysates from melanoma cell lines A375, MeWo, and HS695T showed that four members of the NCBT family of bicarbonate transporters were expressed to varying degrees in the human melanoma cell lines (Fig. 5c), including the electrogenic sodium-bicarbonate cotransporters 1 and 2 (NBCe1 and 2), the electroneutral sodium-bicarbonate cotransporter 1 (NBCn1), and the sodium-dependent chloride/bicarbonate exchanger (NDCBE) (Fig. 5c). The gene discussed is SLC4A8; the disease is melanoma.