In saturation binding studies, [89Zr]Zr-DFO-PD-L1 mAb exhibited high specific binding and affinity for PD-L1 with a Kd of 0.392 ± 0.0481 nmol/L (n = 7) in both MDA-MB231 (moderate/low PD-L1 expression) and HCC-827 cells (moderate; Figure 1A). This evidence concerns the gene CD274 and hepatocellular carcinoma.