SIX1 and neoplasm: Previous research has shown that up‐regulation of Six1 in HCC is associated with a worse tumour grade.26 Using a multivariate analysis, it has also been suggested that Six1 could be an independent risk factor for tumour recurrence, therefore having a significant negative impact on survivability.16, 27 Additionally, compared to those in HCC patients without metastases, Six1 expression levels were high in tissues from patients with metastases.28 All these data provide persuasive evidence to prove the ability of Six1 to facilitate progression and metastasis in HCC (Figure 6H).