The analysis of the variations at each of the HLA-I genes (HLA-A, HLA-B, and HLA-C) in the high and low-quality neoantigen cohorts revealed that at least one HLA-I allele was homozygous in 25.0% (53 of 212) of IDH wild-type GBM containing only low-quality neoantigens but the frequency of HLA-I homozygosity was significantly lower in the high-quality neoantigen group (10.7% or 6 of 56, One-sided Fisher exact test p = 0.0136, Supplementary Table 2). This evidence concerns the gene HLA-B and glioblastoma.