To analyze FSCN1 mRNA and protein expression in TSCC tissues (T) and the adjacent non-carcinoma tissues (ANT), we performed qRT-PCR assay and found that the expression of FSCN1 mRNA was demonstrably higher in T than in ANT in 48 pairs of fresh frozen tissues from TSCC patients (p < 0.0001) (Fig. 4a). The gene discussed is FSCN1; the disease is carcinoma.