The strong association of high PRMT1 expression in human ESCC with enlargement of OV6 cells, and the increased PRMT1 expressed in magnetically isolated esophageal TICs and spheroid formation supporting our assumption that PRMT1 promotes ESCC initiation and progression via the regulation of tumour initiating cells. This evidence concerns the gene PRMT1 and esophageal squamous cell carcinoma.