To evaluate the anti-tumor activity of scFv PD-L1 and scFvFc PD-L1 in a more clinically relevant scenario, we sought to determine the minimal transduction level required for scFv PD-L1 to achieve anti-tumor activity, using different ratios of EMT6 cells maximally pre-transduced with RRV-scFv-PDL1, RRV-scFvFc-PDL1 or RRV-GFP vectors. This evidence concerns the gene CD274 and neoplasm.