In this experiment, EMT6 mouse breast cancer cells maximally infected with RRV-scFv-HF-PDL1, mixed with EMT6 cells maximally infected with RRV-GFP at various ratios were co-cultured to measure bound scFv-HF PD-L1 and unbound PD-L1 on the cell surface using the anti-HA and anti-PD-L1 antibody. Here, CD274 is linked to breast carcinoma.