For tumors that expressed scFv PD-L1, as low as 2% pre-transduced tumor was sufficient to provide survival benefit and the expression of IgG1 Fc as part of the scFvFc PD-L1 intended for improving protein stability or biodistribution of scFvFc PD-L1 appears to be dispensable as it did not improve survival benefit over scFv PD-L1 expressing tumors in all 3 ratios tested. This evidence concerns the gene CD274 and neoplasm.