On the other hand, alterations in NF1, including early termination, were not sufficient to raise the resistance of double-wild cells, such as YUSOC (IC50 157), YUHEF (IC50 257, NF1 pQ853X) and YUTOGS (IC50 347, NF1 p.W336X/E337K), or cells carrying the fusion protein PDE8A-RAF1 YUSIV (IC50 81 nM, NF1 p.L626F), but may had an impact on one BRAFV600K/M melanoma (YUCHIME) displaying extreme resistance (IC50 2,477 nM, NF1 p.K1714N). The gene discussed is PDE8A; the disease is melanoma.