The MYCN non-amplified (non-MNA) SH-SY5Y and MYCN-amplified (MNA) LA-N-5 and IMR-32 NB cell lines were cultured in the presence of JQ1 at different doses for 24, 48 and 72 hours in order to identify optimal conditions enabling cell viability and preventing apoptosis which could compromise the surface expression of ligands. The gene discussed is MYCN; the disease is neuroblastoma.