To investigate the role of gene variants in CHD/heterotaxy diseases, we carried out targeted sequencing on the exonic regions of the following 37 PCD-related candidate genes in 42 CHD patients with heterotaxy: ABCC4, ARMC4, C21orf59, CCDC39, CCDC40, CCDC65, CCDC114, CCDC151, CCNO, DNAAF1, DNAAF2, DNAFF3, DNAAF5, DNAH5, DNAH8, DNAH11, DNAI1, DNAI2, DNAL1, DRC1, DYX1C1, HEATR2, HYDIN, LRRC6, NAT10, NME8, PTGES, PTGES2, PTGES3, PTGER4, PTGS1, PTGS2, RSPH1, RSPH4A, RSPH9, SPAG1 and ZMYND10. This evidence concerns the gene PTGES3 and coronary artery disorder.