CCL7 and cyst: To validate whether the uncharacterized hypothetical proteins from the cyst wall pulldown were novel cyst wall proteins, genes that were not detected from GRAomic data sets (12, 13) (TgME49_230705, TgME49_261650) or had high expression within the M4 in vivo bradyzoite transcriptome data set deposited in ToxoDB (6) (MCP3, TgME49_260520, TgME49_203600, TgME49_319340) were chosen to be expressed in the parasite with a 3xHA epitope tag driven by their native promoters (Fig. 2A).