Because we had evidence that NF-κB-mediated transcription is stimulated by ascites in ovarian cancer cell lines (Lane, unpublished data), and because NF-κB is a downstream effector of several cytokines, we assessed the role of NF-κB in ascites-induced stimulation of MUC16 expression and release. This evidence concerns the gene NFKB1 and ovarian carcinoma.