Two murine anti-tau antibodies (IgG gamma 1), named A and D, recognizing the 50–65 kD PHF triplet banding pattern, which is classical in Alzheimer’s disease (Courade et al., 2018) and an isotype antibody recognizing the human TNFα but not murine TNFα (negative control antibody) were used for immunization study. The gene discussed is TNF; the disease is early-onset autosomal dominant Alzheimer disease.