ITGAM and neoplasm: Flow cytometry analysis on the total cells isolated from subcutaneous B16F10 tumours (gating strategy is described in supplementary information and Fig. S11A) developed in both strains of mice showed that tumours from the immunodeficient NSG mice had a significantly smaller population of TAMs (CD45+ F4/80+ CD11b+) (Fig. S11B) compared to that of the tumours from C57Bl/6 mice, which supports the hypothesis above.