Since Rv1468c-Ub interaction-triggered xenophagy was associated with the recruitment of p62 and LC3 to Mtb, we thus deleted p62 and Atg5 in RAW264.7 macrophages by using CRISPR/Cas9-mediated gene targeting to confirm that this antimycobacterial process was contributed by host selective autophagy, and we found that after infection for 24 h, Mtb ∆Rv1468c and Mtb ∆Rv1468c:Rv1468c L65G exhibited enhanced intracellular viability as compared to WT Mtb or Mtb ∆Rv1468c:Rv1468c in WT RAW264.7 cells, but showed little survival advantage in p62-/- and Atg5-/- RAW264.7 cells (Supplementary Fig. 10a–c). This evidence concerns the gene ATG5 and infection.