SHH and urinary bladder neoplasm: Consistent with the results from BBN-induced tumors, our analyses revealed that the CpG shore of the Shh promoter region in bladder tumor organoids were also hypermethylated, and following the treatment of 5’-azacitidine, the level of methylation was decreased (Figure 1D,E), with significant increases in the expression of Shh (Figure 1F).