Three invasive bladder cancer cell lines, J82, T24 and TCC-SUP, were chosen (Bubeník et al., 1973; Nayak et al., 1977; O'Toole et al., 1978) and analyzed for the level of methylation in the regulatory region of SHH by bisulfite sequencing. The gene discussed is SHH; the disease is urinary bladder cancer.