In agreement with our in vivo findings from tumor-isolated TAMs, DMXAA efficiently activates M2-polarized macrophages, resulting in the highest upregulation of MHC and costimulatory molecules compared to LPS-activated M1-type macrophages and DMXAA single treated bone marrow-derived macrophages (Additional file 3: Figure S3). This evidence concerns the gene HLA-C and neoplasm.