These observations confirm and extend previous studies [14,15,26,38] to clearly indicate that plasma membrane-associated p-p85α (Tyr 508)–p110α complexes are key upstream regulators of Akt and PTEN that mediate the formation of cyclin B1–p-CDK1 (Thr 161) complexes and regulate G2/M phase progression in cancer cells toward the anaerobic glycolytic process. This evidence concerns the gene PTEN and cancer.