Here, multi-genetically modified moDCs (Ad-siSSF DCs) were manufactured based on an adenovirus delivering: (i) secretory flagellin, a Toll-like receptor (TLR)-5 agonist inducing DC maturation; (ii) a survivin-MUC1 fusion protein, two leukemia-associated antigens; and (iii) SOCS1 shRNA, an RNA interference moiety overriding the intracellular immune checkpoint molecule SOCS1 [46]. Here, MUC1 is linked to leukemia.