In the present study, we showed that cisplatin can induce mitochondrial fission and depolarization in HCC cells, which is a precondition of mitophagy.43 Western blot analysis showed that cisplatin activated PINK1/parkin‐mediated mitophagy in HCC cells, which selectively targeted impaired mitochondria for degradation. This evidence concerns the gene PRKN and hepatocellular carcinoma.