To this end, we generated TNFα-stimulated MDA-MB-231:MSC “Contact” co-cultures in which CXCL8 was down-regulated in high efficiency by siRNA in the tumor cells and in the MSCs simultaneously [80–90% efficiency was found in CXCL8 down-regulation by the siRNA, similar to our findings in our parallel study (80); that study also demonstrates which of the cells contributed more to CXCL8 production when the tumor-stroma-inflammation network was established with MDA-MB-231 cells]. Here, CXCL8 is linked to neoplasm.