SGTA and infection: To estimate impact of BST2 KO on MOI, we set-up an infection assay with inGFPt and inmCherry vectors and calculated the relative numbers of double positive cells as outlined above for Figure 4C. In contrast to wt Jurkat cells, where Vpu deletion increased MOI (Figure 5D, two left bars), BST2 KO abolished the difference between MOI levels detected for wt and ΔVpu viruses (Figure 5D, two right bars).