In this study, we used a Salmonella induced colitis model to evaluate the role of SopB in bacterial pathogenesis and employed SopB mutant strain ΔsopB as well as mixed lineage kinase domain-like (MLKL) deficient mice to elucidate the contribution of SopB in manipulating host immune response during bacterial invasion and dissemination in vivo. This evidence concerns the gene MLKL and colitis.