The ROC curve analysis for GD/RA/SLE/SSc patients as compared with healthy controls in CD4+ subset revealed that the methylation levels of the 6 DMS (including cg06872964) had a high AUC value of 0.86, with sensitivity of 0.81 and specificity of 0.80 (Supplementary Figure 15A and Supplementary Table 8). This evidence concerns the gene CD4 and systemic lupus erythematosus.