Having previously identified that the F3 domain of NH36 contains important epitopes involved in the CD4+ lymphocyte mediated protection against visceral leishmaniasis caused by L. (L.)infantum chagasi (38) and against cutaneous leishmaniasis produced by L. (L.)amazonensis (35) we aimed, in this work, to design of a multi-epitope vaccine against leishmaniasis. The gene discussed is CD4; the disease is visceral leishmaniasis.