Noticeably, Silva et al. demonstrated decreased growth of B16F10 melanoma in both therapeutic and prophylactic settings using MHC class I or II restricted melanoma peptides Melan-A and gp100 encapsulated into PLGA NP together with either one or both of the TLR3 and TLR9 ligands polyI:C and CpG (148). This evidence concerns the gene HLA-C and melanoma.