Two options have been used to reproduce hyperglycemic situations in preclinical stroke models, depending on the researchers' requirements: acute hyperglycemia after anesthesia can be mimicked in animals with intravenous infusion of glucose (65); or intravenous administration of streptozotocin and continuous administration of insulin, in which the animals develop hyperglycemia after 1 week (66). This evidence concerns the gene INS and Hyperglycemia.