Here, we compared the levels of mitochondrial Parkin accumulation and LC3-mediated autophagosome formation under basal (untreated) conditions in cardiomyocytes from young (2.5 months), aged (2.5 years), and aged rabbits (2.5 years) with HF induced by combined aortic insufficiency and stenosis. This evidence concerns the gene MAP1LC3A and stricture.