Analysis of the percentages of circulating Th1 (CD4+IFNγ+), Th2 (CD4+IL4+) and the CD4+CD25+CD127low regulatory T cells as well as the Th17 (CD4+IL17A+) cells revealed intriguing data suggesting the suitability of the HFD-dNONcNZO mice for studying obesity-induced immunological alterations and dysfunctional ovarian responses during early gestation in individuals with features of PCOS. This evidence concerns the gene IL4 and obesity due to melanocortin 4 receptor deficiency.