To rule out the possibility that necroptosis is the form of cell death promoting the K+ efflux involved in NLRP3 activation after viral infection, MLKL−/− BMDMs were infected with VSV or EMCV but IL-1β release as well as replication were similar to the infected WT BMDMs (Fig. 5b, c). The gene discussed is NLRP3; the disease is viral infectious disease.