NOTCH2 and neoplasm: The model also predicts high-confidence branching evolution patterns consistent with subclones A1 (PPP2R1A, SYNE2 and AURKA), A2 (ECM2, CHRM5 and TGFB2), and H (NRRK1, AFF4, ECM1, CBX4), and provides an explicit ordering among clonal mutations in PTEN, TBX3 and NOTCH2, which trigger tumour initiation.