Supporting this, studies have shown that adult Dio3KO mice exhibit an increase in T3 concentration, leading to a state of central hypothyroidism and decreased expression of T3-regulated genes (Hernandez et al., 2010, 2012), which is consistent with the data obtained for adult P60 animals in the present study, in which we found no significant difference in the expression of AQP4 between the control and T3-treated animals. This evidence concerns the gene AQP4 and Central hypothyroidism.