Note that N-terminal Shh processing during release is not Bosc-cell specific, because HeLa (adenocarcinoma), Panc1 (pancreatic carcinoma), and MiaPaCa (pancreatic carcinoma) cells can also process the N-terminal Hh peptide during release from the producing cell surface, as indicated by its increased electrophoretic mobility (Supplementary Figure S3). Here, SHH is linked to exocrine pancreatic carcinoma.