We did not observed significant externalization of phosphatidylserine (main marker of early apoptosis, detected by FITC-conjugated Annexin V) during C6 glioma treatment with low (0.1 and 0.5 μg/mL) and high doses of Les-3288 (1.0 μg/mL), suggesting that this compound is a weak inducer of apoptosis. This evidence concerns the gene ANXA5 and glioma.