Interestingly, although FAM129A was more reduced in si-FAM129A-transfected 786-O and ACHN cells (Figs. 4f, 5a, b and 7), more reductions on the invasion and slightly higher reductions on the migration capacities were observed in miR-4521-overexpressing 786-O and ACHN cells (Figs. 5e, f and 6c, d), which was also consistent with more increased TIMP-1 upregulation and MMP2 and MMP9 downregulations resulting in better suppressed invasiveness and metastases of tumor cells. This evidence concerns the gene MMP2 and neoplasm.