USP6NL and neoplasm: Our data showed that siUSP6NL significantly inhibited tumor volume and tumor weight (Fig. 6a, b, all P < 0.01), promoted apoptosis in tumor tissue (Fig. 6c), reduced USP6NL, β-catenin, Cyclin D1 and C-myc while increased P27 when compared with siNC group (Fig. 6d), suggesting the involvement of USP6NL and its potential association with Wnt/β-catenin pathway and C-myc in CRC in vivo.