Our CCK-8 assay also showed that the cell proliferation ability of ccRCC cell lines was significantly decreased in the LV-HHLA2-sh1 (P < 0.0001 in 786-O cells, and P < 0.0001 in ACHN cells) and LV-HHLA2-sh2 (P < 0.0001 in 786-O cells, and P < 0.0001 in ACHN cells) groups compared with the LV-NC group (Fig. 3g, h). This evidence concerns the gene HHLA2 and nonpapillary renal cell carcinoma.